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Addgene

pCold TDP43 WT
(Plasmid #27451)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27451 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCold 1
  • Backbone manufacturer
    Takara
  • Backbone size w/o insert (bp) 4407
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TDP-43
  • Species
    H. sapiens (human)
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)
  • Tags / Fusion Proteins
    • His6 (N terminal on insert)
    • Factor Xa (N terminal on insert)
    • GST (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCold TDP43 WT was a gift from Aaron Gitler (Addgene plasmid # 27451 ; http://n2t.net/addgene:27451 ; RRID:Addgene_27451)

  • For your References section:

    TDP-43 is intrinsically aggregation-prone, and amyotrophic lateral sclerosis-linked mutations accelerate aggregation and increase toxicity. Johnson BS, Snead D, Lee JJ, McCaffery JM, Shorter J, Gitler AD. J Biol Chem. 2009 Jul 24. 284(30):20329-39. 10.1074/jbc.M109.010264 PubMed 19465477
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