pRS416 Gal G294A YFP
(Plasmid #27448)

• Depositing Lab: Aaron Gitler
• Publication: Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-39.

Backbone

• Vector backbone: pRS416GAL
• Backbone size w/o insert (bp): 5000
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TDP-43
• Species: H. sapiens (human)
• Mutation: Glycine 294 mutated to Alanine (G294A)
• Entrez Gene: TARDBP (a.k.a. ALS10, TDP-43)
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pRSGal-Fwd GTTAATATACCTCTATACTTTAACGTCAAGGAGA
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry