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pBT264
(Plasmid #27438)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27438 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2868
  • Vector type
    Mouse Targeting ; tTA dependent

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha, standard LB buffer at 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRE-tdTomato-myc flanked by insulators
  • Species
    Aquorea victoria
  • Insert Size (bp)
    3270
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SwaI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer M13Reverse
  • 3′ sequencing primer M13Forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT264 was a gift from Liqun Luo (Addgene plasmid # 27438 ; http://n2t.net/addgene:27438 ; RRID:Addgene_27438)

  • For your References section:

    Cortical representations of olfactory input by trans-synaptic tracing. Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo L. Nature. 2010 Dec 22. ():. 10.1038/nature09714 PubMed 21179085
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