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Addgene

pAAV-TRE-HTG
(Plasmid #27437)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 27437 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV2-MCS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2887
  • Vector type
    AAV ; adeno-associated viral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    DH5alpha, standard LB buffer,37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    histone-GFP-2A-TVA receptor-2A-rabies glycoprotein
  • Species
    rabies virus
  • Insert Size (bp)
    4175
  • Mutation
    N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Ed Callaway at Salk inst.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TRE-HTG was a gift from Liqun Luo (Addgene plasmid # 27437 ; http://n2t.net/addgene:27437 ; RRID:Addgene_27437)

  • For your References section:

    Cortical representations of olfactory input by trans-synaptic tracing. Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo L. Nature. 2010 Dec 22. ():. 10.1038/nature09714 PubMed 21179085
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