CtkA_300_pET21a
(Plasmid #27429)

• Depositing Lab: SeWon Suh
• Publication: Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. ():.

Backbone

• Vector backbone: pET21a
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cell translocating kinas A
• Alt name: CtkA
• Alt name: Jhp0940
• Species: H. pylori
• Insert Size (bp): 900
• Mutation: residues 301-325 are truncated
• Entrez Gene: jhp0940
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry