Skip to main content
Addgene

pGEX-4T3-ZO-2-1st-PDZ-delta-NLS
(Plasmid #27425)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEX-4T3
  • Backbone manufacturer
    Pharmacia
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zonula Occludens 2
  • Alt name
    ZO-2
  • Alt name
    TJP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    347
  • Mutation
    Deletion of NLS-Nuclear Localization Signal: Nucleotides 313-873, counting A in the ATG start codon as 1, are deleted.
  • GenBank ID
    AAH27592
  • Entrez Gene
    TJP2 (a.k.a. C9DUPq21.11, DFNA51, DUP9q21.11, FHCA1, PFIC4, X104, ZO2)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site XboI (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T3-ZO-2-1st-PDZ-delta-NLS was a gift from Marius Sudol (Addgene plasmid # 27425 ; http://n2t.net/addgene:27425 ; RRID:Addgene_27425)

  • For your References section:

    Functional complexes between YAP2 and ZO-2 are PDZ domain-dependent, and regulate YAP2 nuclear localization and signalling. Oka T, Remue E, Meerschaert K, Vanloo B, Boucherie C, Gfeller D, Bader GD, Sidhu SS, Vandekerckhove J, Gettemans J, Sudol M. Biochem J. 2010 Nov 25. 432(3):461-72. 10.1042/BJ20100870 PubMed 20868367
Related items:
Commonly requested with: