p2Flag CMV2-ZO-2-N
(Plasmid #27418)

• Depositing Lab: Marius Sudol
• Publication: Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Backbone

• Vector backbone: p2xFlag CMV2
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Zonula Occludens 2
• Alt name: ZO-2
• Alt name: TJP2
• Species: H. sapiens (human)
• Insert Size (bp): 982
• Mutation: Deletion of the carboxy-terminus of ZO-2, so only the first 327 amino acids of the protein, which include PDZ1 domain, are expressed. N stands for N-terminal construct. See Figure 3 in the paper for details.
• GenBank ID: AAH27592
• Entrez Gene: TJP2 (a.k.a. C9DUPq21.11, DFNA51, DUP9q21.11, FHCA1, PFIC4, X104, ZO2)
• Tag / Fusion Protein:
  • 2xFlag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: BglII/BamH1 (unknown if destroyed)
• 5′ sequencing primer: CMV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry