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Addgene

pMRS156.2 (miR156 sensor)
(Plasmid #27412)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27412 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCGTAG
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Growth instructions
    MACH-1 strain of E. coli (from Invitrogen)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    miR156 target site
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The miR156 sensor construct was created by cloning miR156 target sites into the control sensor plasmid (Addgene Plasmid # 27405).

miR156 target site in the 5' untranslated region (UTR) of a ubiquitously expressed nuclear-localized GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRS156.2 (miR156 sensor) was a gift from David Bartel (Addgene plasmid # 27412 ; http://n2t.net/addgene:27412 ; RRID:Addgene_27412)
  • For your References section:

    MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis. Nodine MD, Bartel DP. Genes Dev. 2010 Dec 1. 24(23):2678-92. 10.1101/gad.1986710 PubMed 21123653