Skip to main content
Addgene

SPL11p::pCGTAG (SPL11p::NLS-GFP)
(Plasmid #27411)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27411 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCGTAG
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Growth instructions
    MACH-1 strain of E. coli (from Invitrogen)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SPL11 promoter
  • Species
    A. thaliana (mustard weed)
  • Mutation
    SPL11 promoter transcriptionally fused to a nuclear-localized GFP
  • Entrez Gene
    AT1G27360 (a.k.a. F17L21.14, F17L21_14)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SPL11p::NLS-GFP was generated by PCR amplification of 2.2-kb genomic DNA. The resulting amplicon was then cloned into pCR8/GW-TOPO (Invitrogen) and recombined with pCGTAG.

The primers used to create this construct were 5'-AAATCTCGTCCAACT-3' and 5'-TCACGATATGGGTTG-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SPL11p::pCGTAG (SPL11p::NLS-GFP) was a gift from David Bartel (Addgene plasmid # 27411 ; http://n2t.net/addgene:27411 ; RRID:Addgene_27411)

  • For your References section:

    MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis. Nodine MD, Bartel DP. Genes Dev. 2010 Dec 1. 24(23):2678-92. 10.1101/gad.1986710 PubMed 21123653
Related items: