SPL10p::pCGTAG (SPL10p::NLS-GFP)
(Plasmid #27410)

• Depositing Lab: David Bartel
• Publication: Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Backbone

• Vector backbone: pCGTAG
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Growth instructions: MACH-1 strain of E. coli (from Invitrogen)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SPL10 promoter
• Species: A. thaliana (mustard weed)
• Mutation: SPL10 promoter transcriptionally fused to a nuclear-localized GFP
• Entrez Gene: AT1G27370 (a.k.a. F17L21.15, F17L21_15)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SPL10p::NLS-GFP was generated by PCR amplification of 1.9-kb genomic DNA. The resulting amplicon was then cloned into pCR8/GW-TOPO (Invitrogen) and recombined with pCGTAG.

The primers used to create this construct were 5'-TCATCATCTTTCACG-3' and 5'-TCAAGATGTGGGTTG-3'.

Please note that Addgene's sequencing results identified single nucleotide mismatches at bp# 11592, 11985 and 12403 when compared to the full plasmid sequence provided by the depositing laboratory. These mismatches are not known to affect the plasmid function.