UB13p::dsSPL10 v01 (SPL10-RNAi)
(Plasmid #27409)

• Depositing Lab: David Bartel
• Publication: Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Backbone

• Vector backbone: UBI3pTpFSPGW-HYGv02
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Growth instructions: MACH-1 strain of E. coli (from Invitrogen)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SPL10 inverted
• Alt name: SPL10
• Species: A. thaliana (mustard weed)
• Entrez Gene: AT1G27370 (a.k.a. F17L21.15, F17L21_15)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: M13 forward 20

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SPL10-RNAi constructs were generated by amplifying 0.6kb
of the SPL10 CDS from cDNA prepared from inflorescence tissue total RNA isolated using TRIzol reagent (Invitrogen) and reverse-transcribed with oligo(dT) primers using SuperScript
III Reverse Transcriptase (Invitrogen). The resulting amplicons were then cloned into pCR8/GW-TOPO (Invitrogen) and then recombined with UBI3p::pFSPGW-HYGv02 to create an inverted repeat of the SPL10 cDNA fragment under the control
of the UBI3 promoter. For details regarding the UBI3p::pFSPGW-HYGv02 vector, please see the article.