pSPL10p::SPL10-GFP
(Plasmid #27406)

• Depositing Lab: David Bartel
• Publication: Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Backbone

• Vector backbone: KAN-GWR-GFP
• Vector type: used to create transgenic lines

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Growth instructions: MACH-1 strain of E. coli (from Invitrogen)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SPL10
• Species: A. thaliana (mustard weed)
• Entrez Gene: AT1G27370 (a.k.a. F17L21.15, F17L21_15)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: GFP Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SPL10p::SPL10-GFP constructs were generated by cloning genomic fragments, including 1.7 kb upstream of and including the SPL10 coding sequence (CDS), into pENTR/D-TOPO (Invitrogen), and then recombining these plasmids with Gateway compatible pBIB-KAN-GWR-GFP plasmids using LR clonase (Invitrogen).