pMRS001.2-GFP (control sensor)
(Plasmid
#27405)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27405 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCGTAG
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Growth instructionsMACH-1 strain of E. coli (from Invitrogen)
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The control sensor construct was created by cloning the potato UBI3 promoter into pENTR/D-TOPO (Invitrogen). The resulting plasmid was then recombined with pCGTAG. The promoter results in a ubiquitously expressed nuclear localized GFP.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRS001.2-GFP (control sensor) was a gift from David Bartel (Addgene plasmid # 27405 ; http://n2t.net/addgene:27405 ; RRID:Addgene_27405) -
For your References section:
MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis. Nodine MD, Bartel DP. Genes Dev. 2010 Dec 1. 24(23):2678-92. 10.1101/gad.1986710 PubMed 21123653
