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pETO18G-H7C/R86C
(Plasmid #27403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27403 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET16b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    leuT
  • Species
    Aquifex aeolicus
  • Insert Size (bp)
    1607
  • Mutation
    Changes His 7 to Cys and Arg 86 to Cys
  • Tags / Fusion Proteins
    • HisX8 (C terminal on insert)
    • Thrombin (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon-optimized leuT of Aquifex aeolicus (position 107 – 1699; including a 8-His tag, a thrombin cleavage site, and codon changes at position 125-127 [CAT]His to [TGT]Cys, 362 -364 to [CGC]Arg to[TGC]Cys)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETO18G-H7C/R86C was a gift from Jonathan Javitch (Addgene plasmid # 27403 ; http://n2t.net/addgene:27403 ; RRID:Addgene_27403)

  • For your References section:

    Single-molecule dynamics of gating in a neurotransmitter transporter homologue. Zhao Y, Terry D, Shi L, Weinstein H, Blanchard SC, Javitch JA. Nature. 2010 May 13. 465(7295):188-93. 10.1038/nature09057 PubMed 20463731
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