pETO18G-AP
(Plasmid
#27402)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27402 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET16b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameleuT
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SpeciesAquifex aeolicus
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Insert Size (bp)1607
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Tags
/ Fusion Proteins
- HisX8 (C terminal on insert)
- Thrombin (C terminal on insert)
- Biotin Acceptor Domain (BAD) (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Codon-optimized leuT of Aquifex aeolicus (position 107 – 1735; including a 8-His tag, a thrombin cleavage site, and a biotin acceptor domain [1697 – 1750; introduced by PCR coding region at the 3’ end of the gene; in this context a BamHI site was removed at position 511)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETO18G-AP was a gift from Jonathan Javitch (Addgene plasmid # 27402 ; http://n2t.net/addgene:27402 ; RRID:Addgene_27402) -
For your References section:
Single-molecule dynamics of gating in a neurotransmitter transporter homologue. Zhao Y, Terry D, Shi L, Weinstein H, Blanchard SC, Javitch JA. Nature. 2010 May 13. 465(7295):188-93. 10.1038/nature09057 PubMed 20463731
Map uploaded by the depositor.
