pQO18 TEV-L400C
(Plasmid
#27400)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQE
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Backbone manufacturerQiagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameleuT
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SpeciesAquifex aeolicus
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Insert Size (bp)1607
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MutationChanged Leu 400 to Cys
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Tags
/ Fusion Proteins
- HisX10 (N terminal on insert)
- TEV (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pQE-Fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The leuT gene is codon-optimized for the expression in E. coli and designed as cassette version that contains unique restriction sites in the pQE vector background and was first described in Shi, Quick et al., 2008.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQO18 TEV-L400C was a gift from Jonathan Javitch (Addgene plasmid # 27400 ; http://n2t.net/addgene:27400 ; RRID:Addgene_27400) -
For your References section:
Single-molecule dynamics of gating in a neurotransmitter transporter homologue. Zhao Y, Terry D, Shi L, Weinstein H, Blanchard SC, Javitch JA. Nature. 2010 May 13. 465(7295):188-93. 10.1038/nature09057 PubMed 20463731