Skip to main content
Addgene

pQO18-TEV
(Plasmid #27399)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pQE
  • Backbone manufacturer
    Qiagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    leuT
  • Species
    Aquifex aeolicus
  • Insert Size (bp)
    1607
  • Tags / Fusion Proteins
    • HisX10 (N terminal on insert)
    • TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pQE-Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The leuT gene is codon-optimized for the expression in E. coli and designed as cassette version that contains unique restriction sites in the pQE vector background and was first described in Shi, Quick et al., 2008.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQO18-TEV was a gift from Jonathan Javitch (Addgene plasmid # 27399 ; http://n2t.net/addgene:27399 ; RRID:Addgene_27399)

  • For your References section:

    Single-molecule dynamics of gating in a neurotransmitter transporter homologue. Zhao Y, Terry D, Shi L, Weinstein H, Blanchard SC, Javitch JA. Nature. 2010 May 13. 465(7295):188-93. 10.1038/nature09057 PubMed 20463731
Related items:
Commonly requested with: