pBB540
(Plasmid #27393)

• Depositing Lab: Bernd Bukau
• Publication: de Marco et al BMC Biotechnol. 2007 . 7():32.

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 7194
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: grpE, clpB
• Species: E. coli
• Insert Size (bp): 7194

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Dr. Bernd Bukau, ZMBH, University of Heidelberg
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.

de Marco A.

Nat Protoc. 2007;2(10):2632-9.