pBB535
(Plasmid #27392)

• Depositing Lab: Bernd Bukau
• Publication: de Marco et al BMC Biotechnol. 2007 . 7():32.

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 7072
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: dnaK, dnaJ
• Species: E. coli
• Entrez Gene: dnaJ (a.k.a. b0015, ECK0015, groP, grpC)
• Entrez Gene: dnaK (a.k.a. b0014, ECK0014, groPAB, groPC, groPF, grpC, grpF, seg)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results detect a single nucleotide insertion compared to depositor's sequence in the vector backbone. The plasmid expresses the expected functional protein.