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Addgene

pBB530
(Plasmid #27391)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27391 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 4493
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    grpE
  • Species
    E. coli

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Bernd Bukau, ZMBH University of Heidelberg

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.

de Marco A.

Nat Protoc. 2007;2(10):2632-9.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBB530 was a gift from Bernd Bukau (Addgene plasmid # 27391 ; http://n2t.net/addgene:27391 ; RRID:Addgene_27391)
  • For your References section:

    Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli. de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B. BMC Biotechnol. 2007 . 7():32. 10.1186/1472-6750-7-32 PubMed 17565681
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