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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27391 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
- Backbone size w/o insert (bp) 4493
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namegrpE
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SpeciesE. coli
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Bernd Bukau, ZMBH University of Heidelberg
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBB530 was a gift from Bernd Bukau (Addgene plasmid # 27391 ; http://n2t.net/addgene:27391 ; RRID:Addgene_27391) -
For your References section:
Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli. de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B. BMC Biotechnol. 2007 . 7():32. 10.1186/1472-6750-7-32 PubMed 17565681