pMo168
(Plasmid #27389)

• Depositing Lab: Martin Voskuil
• Publication: Hamad et al Gene. 2009 Feb 1. 430(1-2):123-31.

Backbone

• Vector backbone: pBBR1
• Backbone size w/o insert (bp): 7108
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): JM109
• Growth instructions: Use JM109 bacterial strain; Grow at 30C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: pMo168
• Species: E. coli
• Mutation: A replicative vector for gene expression in Burkholderia
• GenBank ID: EU862244

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MCS1: ApaI, NheI, BglII, SmaI, HindIII (not destroyed)
• 3′ cloning site: MCS2: PstI, BamHI, XbaI, SphI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp# 5987 and a 5 nucleotide deletion at bp# 6962-6966, when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing scientist, these differences do not affect the function of the plasmid.