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6mSPL11F15R14::pBIB-BAS (6mSPL11)
(Plasmid #27387)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27387 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBIB-KAN-GW
  • Vector type
    used to create transgenic lines

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Growth instructions
    MACH-1 strain of E. coli (from Invitrogen)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SPL11 mutant
  • Alt name
    SPL11
  • Species
    A. thaliana (mustard weed)
  • Mutation
    miR156 target sites of SPL11 mutated
  • Entrez Gene
    AT1G27360 (a.k.a. F17L21.14, F17L21_14)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Created by cloning 5.5-kb (including 2.7 kb upstream of
CDS, CDS, and 1.3 kb downstream from CDS) genomic fragment in pENTR/D-TOPO (Invitrogen). The Quick-
Change Lightning Site-Directed Mutagenesis kit (Stratagene) was used on the resulting plasmids to disrupt miR156-binding sites (primers 5'-AATCTCAAGATATCCACCGTGCACTGTCATTGCTCTCAACCTCTTCGGATCCCCTGG-3' and 5'-CCAGGGGATCCGAAGAGGTTGAGAGCAATGACAGTGCACGGTGGATATCTTGAGATT-3').

This plasmid was recombined with pBIB-KAN-GW using LR clonase (Invitrogen).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6mSPL11F15R14::pBIB-BAS (6mSPL11) was a gift from David Bartel (Addgene plasmid # 27387 ; http://n2t.net/addgene:27387 ; RRID:Addgene_27387)

  • For your References section:

    MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis. Nodine MD, Bartel DP. Genes Dev. 2010 Dec 1. 24(23):2678-92. 10.1101/gad.1986710 PubMed 21123653
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