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Addgene

plenti6.3/hCx43-stop
(Plasmid #27383)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27383 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    plenti6.3/V5-DEST
  • Backbone size w/o insert (bp) 9387
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gja1
  • Alt name
    Connexin 43
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1149
  • GenBank ID
    NM_000165
  • Entrez Gene
    GJA1 (a.k.a. AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA)
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 gateway reaction site (not destroyed)
  • 3′ cloning site attR2 gateway reaction site (not destroyed)
  • 5′ sequencing primer sequence
  • 3′ sequencing primer sequence
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Open Biosystems
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plenti6.3/hCx43-stop was a gift from Robin Shaw (Addgene plasmid # 27383 ; http://n2t.net/addgene:27383 ; RRID:Addgene_27383)

  • For your References section:

    Limited forward trafficking of connexin 43 reduces cell-cell coupling in stressed human and mouse myocardium. Smyth JW, Hong TT, Gao D, Vogan JM, Jensen BC, Fong TS, Simpson PC, Stainier DY, Chi NC, Shaw RM. J Clin Invest. 2010 Jan 4. 120(1):266-79. 10.1172/JCI39740 PubMed 20038810
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