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Addgene

FLAG-mCep290
(Plasmid #27381)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27381 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFLAG-CMV2
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cep290
  • Alt name
    centrosomal protein 290
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    7557
  • Entrez Gene
    Cep290 (a.k.a. BC004690, Np, Nphp6, b2b1454C, b2b1454Clo, b2b1752C, b2b1752Clo)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-mCep290 was a gift from Joseph Gleeson (Addgene plasmid # 27381 ; http://n2t.net/addgene:27381 ; RRID:Addgene_27381)

  • For your References section:

    Mutations in CEP290, which encodes a centrosomal protein, cause pleiotropic forms of Joubert syndrome. Valente EM, Silhavy JL, Brancati F, Barrano G, Krishnaswami SR, Castori M, Lancaster MA, Boltshauser E, Boccone L, Al-Gazali L, Fazzi E, Signorini S, Louie CM, Bellacchio E, Bertini E, Dallapiccola B, Gleeson JG. Nat Genet. 2006 Jun . 38(6):623-5. 10.1038/ng1805 PubMed 16682970
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