pCMV-Flag YAP127/128/131/381A
(Plasmid #27378)

• Depositing Lab: Kunliang Guan
• Publication: Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Backbone

• Vector backbone: pCMV
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YAP
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: S127/128/131/381A
• Entrez Gene: YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
• Tag / Fusion Protein:
  • 2xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry