pGMCtN-P38-sspBmyc
(Plasmid
#27375)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27375 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDE43-MCtNq1
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Backbone manufacturerN/A
- Backbone size w/o insert (bp) 3725
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Vector typeBacterial Expression ; Gateway Destination Vector
Growth in Bacteria
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Bacterial Resistance(s)Nourseothricin (clonNat), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsMACH 1, 37C
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesspBmyc
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Insert Size (bp)1072
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MutationN/A
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGMCtN-P38-sspBmyc was a gift from Dirk Schnappinger (Addgene plasmid # 27375 ; http://n2t.net/addgene:27375 ; RRID:Addgene_27375) -
For your References section:
Improved tetracycline repressors for gene silencing in mycobacteria. Klotzsche M, Ehrt S, Schnappinger D. Nucleic Acids Res. 2009 Jan 27. ():. 10.1093/nar/gkp015 PubMed 19174563
Map uploaded by the depositor.
