pCMV-flag S127A YAP
(Plasmid #27370)

• Depositing Lab: Kunliang Guan
• Publication: Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Backbone

• Vector backbone: pCMV
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YAP
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: S127A ( major phosphorylation site in YAP2)
• Entrez Gene: YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
• Tag / Fusion Protein:
  • flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCMV-Flag-YAP2 was obtained from Marius Sudol.
• Articles Citing this Plasmid:
  • 28 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry