pDO23A
(Plasmid
#27367)
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27367 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepDONRP2R-P3
-
Backbone manufacturerGateway(R) (Invitrogen)
- Backbone size w/o insert (bp) 3012
-
Vector typeBacterial Expression ; Gateway Donor Vector
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DB3.1
-
Growth instructionsccdB Anti-toxin strain (e.g. DB3.1), 37C
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namepUC/ori, bla
-
Alt nameori, AmpR
-
Insert Size (bp)1813
-
MutationN/A
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site PciI (unknown if destroyed)
- 3′ cloning site ZraI (Insert), EcoRV (Vector) (unknown if destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDO23A was a gift from Dirk Schnappinger (Addgene plasmid # 27367 ; http://n2t.net/addgene:27367 ; RRID:Addgene_27367) -
For your References section:
Simultaneous analysis of multiple Mycobacterium tuberculosis knockdown mutants in vitro and in vivo. Blumenthal A, Trujillo C, Ehrt S, Schnappinger D. PLoS One. 2010 Dec 22;5(12):e15667. doi: 10.1371/journal.pone.0015667. 10.1371/journal.pone.0015667 PubMed 21203517
Map uploaded by the depositor.
