pEN12A-P1
(Plasmid
#27366)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27366 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDO12A
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Backbone manufacturerN/A
- Backbone size w/o insert (bp) 2590
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Vector typeBacterial Expression ; Gateway Donor Vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsMACH 1, 37C
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePmyc1tetO
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Alt namepEN12A-Pmyc1tetO
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Insert Size (bp)394
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MutationN/A
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEN12A-P1 was a gift from Dirk Schnappinger (Addgene plasmid # 27366 ; http://n2t.net/addgene:27366 ; RRID:Addgene_27366) -
For your References section:
Simultaneous analysis of multiple Mycobacterium tuberculosis knockdown mutants in vitro and in vivo. Blumenthal A, Trujillo C, Ehrt S, Schnappinger D. PLoS One. 2010 Dec 22;5(12):e15667. doi: 10.1371/journal.pone.0015667. 10.1371/journal.pone.0015667 PubMed 21203517
Map uploaded by the depositor.
