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Addgene

pEN41A-T10M
(Plasmid #27365)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27365 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDO41A
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 2695
  • Vector type
    Bacterial Expression ; Gateway Donor Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    MACH 1, 37C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tetR(sB)
  • Alt name
    N/A
  • Insert Size (bp)
    1009
  • Mutation
    N/A

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's sequencing results found a single nucleotide mismatch at bp# 2561 when compared to the full sequence information provided by the depositing laboratory.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN41A-T10M was a gift from Dirk Schnappinger (Addgene plasmid # 27365 ; http://n2t.net/addgene:27365 ; RRID:Addgene_27365)

  • For your References section:

    Simultaneous analysis of multiple Mycobacterium tuberculosis knockdown mutants in vitro and in vivo. Blumenthal A, Trujillo C, Ehrt S, Schnappinger D. PLoS One. 2010 Dec 22;5(12):e15667. doi: 10.1371/journal.pone.0015667. 10.1371/journal.pone.0015667 PubMed 21203517
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