pGMCSq17-10M-0X
(Plasmid
#27364)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27364 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDE43-MCSq17
- Backbone size w/o insert (bp) 5127
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Vector typeBacterial Expression ; Gateway Destination Vector
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsMACH 1, 37C
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametetR(sB)
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Alt nameN/A
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Insert Size (bp)1527
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MutationN/A
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGMCSq17-10M-0X was a gift from Dirk Schnappinger (Addgene plasmid # 27364 ; http://n2t.net/addgene:27364 ; RRID:Addgene_27364) -
For your References section:
Simultaneous analysis of multiple Mycobacterium tuberculosis knockdown mutants in vitro and in vivo. Blumenthal A, Trujillo C, Ehrt S, Schnappinger D. PLoS One. 2010 Dec 22;5(12):e15667. doi: 10.1371/journal.pone.0015667. 10.1371/journal.pone.0015667 PubMed 21203517