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Addgene

pDE43-MCSq22
(Plasmid #27335)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27335 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTCS-2M1-icl2
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 4522
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    ccdB Anti-toxin strain (e.g. DB3.1), 37C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    qtag22
  • Alt name
    N/A
  • Insert Size (bp)
    255
  • Mutation
    N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site MluI (unknown if destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDE43-MCSq22 was a gift from Dirk Schnappinger (Addgene plasmid # 27335 ; http://n2t.net/addgene:27335 ; RRID:Addgene_27335)

  • For your References section:

    Simultaneous analysis of multiple Mycobacterium tuberculosis knockdown mutants in vitro and in vivo. Blumenthal A, Trujillo C, Ehrt S, Schnappinger D. PLoS One. 2010 Dec 22;5(12):e15667. doi: 10.1371/journal.pone.0015667. 10.1371/journal.pone.0015667 PubMed 21203517
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