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Addgene

FC858
(Plasmid #27328)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27328 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RS406
  • Backbone size w/o insert (bp) 4384
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDH1
  • Alt name
    RS406-CDH1-m11-HOcs
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    11 Phosphorylation site point mutations: T12A S16A S42A T157A S169A T173A T176A S227A S239A S418A S436A
  • Entrez Gene
    CDH1 (a.k.a. YGL003C, HCT1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FC858 was a gift from Fred Cross (Addgene plasmid # 27328 ; http://n2t.net/addgene:27328 ; RRID:Addgene_27328)

  • For your References section:

    Efficient and rapid exact gene replacement without selection. Cross FR, Pecani K. Yeast. 2010 Oct 22. ():. 10.1002/yea.1822 PubMed 20973105
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