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Addgene

FC857
(Plasmid #27327)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27327 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RS406
  • Backbone size w/o insert (bp) 4384
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDH1
  • Alt name
    RS406-CDH1-HOcs
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    C-terminal truncation of CDH1 at its BamHI site
  • Entrez Gene
    CDH1 (a.k.a. YGL003C, HCT1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FC857 was a gift from Fred Cross (Addgene plasmid # 27327 ; http://n2t.net/addgene:27327 ; RRID:Addgene_27327)

  • For your References section:

    Efficient and rapid exact gene replacement without selection. Cross FR, Pecani K. Yeast. 2010 Oct 22. ():. 10.1002/yea.1822 PubMed 20973105
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