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Addgene

pFC663
(Plasmid #27324)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27324 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RS406
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SIC1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Contains insertion relative to SGDB sequence of GAA to change sequence E129EEEEGE136 to E129EEEEEGE137.
  • Entrez Gene
    SIC1 (a.k.a. YLR079W, BYC1, SDB25)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13/pUC-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFC663 was a gift from Fred Cross (Addgene plasmid # 27324 ; http://n2t.net/addgene:27324 ; RRID:Addgene_27324)

  • For your References section:

    Phosphorylation of the Sic1 inhibitor of B-type cyclins in Saccharomyces cerevisiae is not essential but contributes to cell cycle robustness. Cross FR, Schroeder L, Bean JM. Genetics. 2007 Jul . 176(3):1541-55. 10.1534/genetics.107.073494 PubMed 17483408
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