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pSport6/FLAG-Wwtr1(Taz)
(Plasmid #27318)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27318 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-BOS
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Taz
  • Alt name
    WwtrI
  • Alt name
    G4.5
  • Alt name
    XAP-2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1636
  • GenBank ID
    AW107266
  • Entrez Gene
    Wwtr1 (a.k.a. 2310058J06Rik, 2610021I22Rik, Taz)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The FLAG-Taz insert originated from pEF-BOS/N-FLAG-Taz (TAZ: a novel transcriptional co-activator regulated by interactions with 14-3-3 and PDZ domain proteins. Kanai F et al. (EMBO J. 2000 Dec 15. 19(24):6778-91.)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSport6/FLAG-Wwtr1(Taz) was a gift from Jonathan Epstein (Addgene plasmid # 27318 ; http://n2t.net/addgene:27318 ; RRID:Addgene_27318)
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