Integrin alpha5-AHCys
(Plasmid #27313)

• Depositing Lab: Timothy Springer
• Publication: Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

Backbone

• Vector backbone: pEF1/V5-HisA
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: integrin alpha 5
• Species: H. sapiens (human)
• Insert Size (bp): 3700
• Mutation: integrin alpha5 (1-954) + AHCys
• Entrez Gene: ITGA5 (a.k.a. CD49e, FNRA, VLA-5, VLA5A)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: EF1a-Fwd
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a minor discrepancy between BLAST truncation position and depositor's truncation position. The discrepancies could be the secretion peptide that the depositing lab does not include in their numbering, or the C-terminal tags (that's about 40 aa.)