pDEST/N1-hBIN1-GFP
(Plasmid #27305)

• Depositing Lab: Robin Shaw
• Publication: Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

Backbone

• Vector backbone: pDEST/N1-GFP
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: bridging integrator 1 (BIN1), transcript variant 8
• Species: H. sapiens (human)
• Insert Size (bp): 1362
• Entrez Gene: BIN1 (a.k.a. AMPH2, AMPHL, CNM2, SH3P9)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attR1 gateway reaction site (not destroyed)
• 3′ cloning site: attR2 gateway reaction site (not destroyed)
• 5′ sequencing primer: sequence

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We did not originally clone this gene. We ordered human BIN1 (transcript variance 8) from origene and cloned it into our gateway destination vector pDEST/N1-GFP. The N1-GFP vector is originally ordered from Clonetech and we converted it into a gateway destination vector.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry