pMIGR AMPK KD
(Plasmid #27296)

• Depositing Lab: Morris Birnbaum
• Publication: Birnbaum lab plasmids (unpublished)

Backbone

• Vector backbone: pMIGR
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 8000
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AMP Acticated Protein Kinase
• Species: R. norvegicus (rat)
• Insert Size (bp): 2000
• Mutation: Kinase Dead = Lys 45 to Arg
• Entrez Gene: Prkaa2 (a.k.a. Ampk, Ampka2)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLXSN 5

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: Peter Leland Univ of Wisconsin
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry