pEF1-alpha-V
(Plasmid #27290)

• Depositing Lab: Timothy Springer
• Publication: Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Backbone

• Vector backbone: pEF1/V5-HisA
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: integrin alpha V
• Alt name: CD51
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Mutation: Isoform 2
• GenBank ID: EU332844
• Entrez Gene: ITGAV (a.k.a. CD51, MSK8, VNRA, VTNR)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: Not I (*note two sites are present) (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry