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Addgene

pcDNA3.1-beta-3
(Plasmid #27289)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27289 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/Myc-His(+)A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    integrin beta 3
  • Alt name
    GPIIIa, CD61
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2600
  • Mutation
    n/a
  • GenBank ID
    NM_000212
  • Entrez Gene
    ITGB3 (a.k.a. BDPLT16, BDPLT2, BDPLT24, CD61, GP3A, GPIIIa, GT, GT2)
  • Tags / Fusion Proteins
    • Myc (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-beta-3 was a gift from Timothy Springer (Addgene plasmid # 27289 ; http://n2t.net/addgene:27289 ; RRID:Addgene_27289)

  • For your References section:

    Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling. Takagi J, Petre BM, Walz T, Springer TA. Cell. 2002 Sep 6. 110(5):599-11. 10.1016/S0092-8674(02)00935-2 PubMed 12230977
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