LRP6-m5-pCS2-VSVG
(Plasmid #27283)

• Depositing Lab: Xi He
• Publication: Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: xenopus injection

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: low density lipoprotein receptor-related protein 6
• Alt name: LRP6
• Species: H. sapiens (human)
• Insert Size (bp): 5013
• Mutation: Changed S/T of all five PPP(S/T)P motifs to Alanine. 2 of of the 5 sites deviate slightly from the consensus sequence (there is one PPAAP and one CPPAP)
• Entrez Gene: LRP6 (a.k.a. ADCAD2, STHAG7)
• Tag / Fusion Protein:
  • VSVG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: Xbal (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Dominant inhibitor of Wnt function, similar to LRPT-dC