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Addgene

LRP6N-IgG-pCS2
(Plasmid #27279)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27279 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    xenopus injection

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    low density lipoprotein receptor-related protein 6
  • Alt name
    LRP6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4918
  • Entrez Gene
    LRP6 (a.k.a. ADCAD2, STHAG7)
  • Tag / Fusion Protein
    • IgG Fc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Extracellular domain of human LRP6 tagged with Fc
Use Asp718 to linearize DNA for in vitro transcription by SP6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LRP6N-IgG-pCS2 was a gift from Xi He (Addgene plasmid # 27279 ; http://n2t.net/addgene:27279 ; RRID:Addgene_27279)

  • For your References section:

    LDL-receptor-related proteins in Wnt signal transduction. Tamai K, Semenov M, Kato Y, Spokony R, Liu C, Katsuyama Y, Hess F, Saint-Jeannet JP, He X. Nature. 2000 Sep 28. 407(6803):530-5. 10.1038/35035117 PubMed 11029007
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