LRP6N-IgG-pCS2
(Plasmid #27279)

• Depositing Lab: Xi He
• Publication: Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: xenopus injection

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: low density lipoprotein receptor-related protein 6
• Alt name: LRP6
• Species: H. sapiens (human)
• Insert Size (bp): 4918
• Entrez Gene: LRP6 (a.k.a. ADCAD2, STHAG7)
• Tag / Fusion Protein:
  • IgG Fc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SnaBI (destroyed during cloning)
• 5′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Extracellular domain of human LRP6 tagged with Fc
Use Asp718 to linearize DNA for in vitro transcription by SP6