pSG-3'Hu-RelA
(Plasmid
#27276)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27276 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSG424
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 3396
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRelA
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SpeciesH. sapiens (human)
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Insert Size (bp)792
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MutationContains only the transactivation domain (aa 292-551)
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Entrez GeneRELA (a.k.a. AIF3BL3, CMCU, NFKB3, p65)
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Tag
/ Fusion Protein
- GAL4 (aa1-147) (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer Gal4 N-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG-3'Hu-RelA was a gift from Thomas Gilmore (Addgene plasmid # 27276 ; http://n2t.net/addgene:27276 ; RRID:Addgene_27276) -
For your References section:
GAL4-I kappa B alpha and GAL4-I kappa B gamma activate transcription by different mechanisms. Morin PJ, Subramanian GS, Gilmore TD. Nucleic Acids Res. 1993 May 11. 21(9):2157-63. 10.1093/nar/21.9.2157 PubMed 8502557