pcDNA-RELΔ424-490
(Plasmid #27267)

• Depositing Lab: Thomas Gilmore
• Publication: Starczynowski et al Oncogene. 2007 Apr 26. 26(19):2685-94.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: REL
• Alt name: RELdeltaTAD1
• Species: H. sapiens (human)
• Insert Size (bp): 1563
• Mutation: Deletion of transactivation domain I (aa 425-490)
• Entrez Gene: REL (a.k.a. C-Rel, HIVEN86A, IMD92)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results confirmed N424 is present in the REL sequence. Despite the plasmid name, amino acids 425-490 of REL have been deleted. This plasmid is exactly as created by the depositing laboratory and as described in the associated publication listed below and in Chin et al., Oncogene. 2009 May 21;28(20):2100-11.