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Addgene

pcDNA-FLAG-RELΔRID
(Plasmid #27265)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27265 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    REL
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of aa 323-422
  • Entrez Gene
    REL (a.k.a. C-Rel, HIVEN86A, IMD92)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Clone starts at aa 2.

Please note that Addgene's sequencing results identified an additional Valine residue in-between I322 and D423 as a result of the cloning process.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-FLAG-RELΔRID was a gift from Thomas Gilmore (Addgene plasmid # 27265 ; http://n2t.net/addgene:27265 ; RRID:Addgene_27265)

  • For your References section:

    Deletion analysis and alternative splicing define a transactivation inhibitory domain in human oncoprotein REL. Leeman JR, Weniger MA, Barth TF, Gilmore TD. Oncogene. 2008 Dec 4. 27(53):6770-81. 10.1038/onc.2008.284 PubMed 18695674
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