pcDNA-FLAG-Nv-NF-kB-Cys
(Plasmid
#27260)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27260 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNv-NF-kB
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SpeciesNematostella vectensis
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Insert Size (bp)1317
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Mutation"Cys allele" = Cysteine at aa 67
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Clone starts at aa 2.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-FLAG-Nv-NF-kB-Cys was a gift from Thomas Gilmore (Addgene plasmid # 27260 ; http://n2t.net/addgene:27260 ; RRID:Addgene_27260) -
For your References section:
Two alleles of NF-kappaB in the sea anemone Nematostella vectensis are widely dispersed in nature and encode proteins with distinct activities. Sullivan JC, Wolenski FS, Reitzel AM, French CE, Traylor-Knowles N, Gilmore TD, Finnerty JR. PLoS One. 2009 . 4(10):e7311. 10.1371/journal.pone.0007311 PubMed 19806194