LRP6 dC-pCS2
(Plasmid
#27258)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27258 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeMammalian Expression ; xenopus injection
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namelow density lipoprotein receptor-related protein 6
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Alt nameLRP6
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SpeciesH. sapiens (human)
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Insert Size (bp)4353
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Mutationcarboxyl intracellular region deleted Residue 1440 to internal XhoI site of gene deleted
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Entrez GeneLRP6 (a.k.a. ADCAD2, STHAG7)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site Xbal (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linearize with Asp718 for in vitro transcription by SP6
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LRP6 dC-pCS2 was a gift from Xi He (Addgene plasmid # 27258 ; http://n2t.net/addgene:27258 ; RRID:Addgene_27258) -
For your References section:
LDL-receptor-related proteins in Wnt signal transduction. Tamai K, Semenov M, Kato Y, Spokony R, Liu C, Katsuyama Y, Hess F, Saint-Jeannet JP, He X. Nature. 2000 Sep 28. 407(6803):530-5. 10.1038/35035117 PubMed 11029007