SG-3'REL
(Plasmid
#27257)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27257 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSG424
- Backbone size w/o insert (bp) 3396
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameREL
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SpeciesH. sapiens (human)
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Insert Size (bp)927
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MutationContains aa 278-587 of REL
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Entrez GeneREL (a.k.a. C-Rel, HIVEN86A, IMD92)
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Tag
/ Fusion Protein
- GAL4 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer Gal4 N-term
- 3′ sequencing primer pBRRevBam_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SG-3'REL was a gift from Thomas Gilmore (Addgene plasmid # 27257 ; http://n2t.net/addgene:27257 ; RRID:Addgene_27257) -
For your References section:
Deletion of either C-terminal transactivation subdomain enhances the in vitro transforming activity of human transcription factor REL in chicken spleen cells. Starczynowski DT, Reynolds JG, Gilmore TD. Oncogene. 2003 Oct 9. 22(44):6928-36. 10.1038/sj.onc.1206801 PubMed 14534540