pcDNA-FLAG-Mo-Trip6
(Plasmid
#27254)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27254 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTrip6
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SpeciesM. musculus (mouse)
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Insert Size (bp)1434
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Entrez GeneTrip6
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Trip6 cDNA starts at aa 2.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-FLAG-Mo-Trip6 was a gift from Thomas Gilmore (Addgene plasmid # 27254 ; http://n2t.net/addgene:27254 ; RRID:Addgene_27254) -
For your References section:
Characterization of mouse Trip6: a putative intracellular signaling protein. Wang Y, Dooher JE, Koedood Zhao M, Gilmore TD. Gene. 1999 Jul 8. 234(2):403-9. 10.1016/S0378-1119(99)00168-7 PubMed 10395914